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    Bio-Rad clarity max western ecl substrate kit bio rad
    Clarity Max Western Ecl Substrate Kit Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clarity+western+ecl+substrate+kit+bio+rad/Clarity+Max+Western+ECL+Substrate/pmc12895380-295-7-13
    Average 97 stars, based on 2720 article reviews
    clarity max western ecl substrate kit bio rad - by Bioz Stars, 2026-09
    97/100 stars

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    Article Title: CD31 signaling promotes the detachment at the uropod of extravasating neutrophils allowing their migration to sites of inflammation
    Article Snippet: or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant human ICAM- 1 R&D Systems Cat# ADP4 20 μg/ml Peptide, recombinant protein Recombinant mouse laminin a4 R&D Systems Cat# 3837- A4 20 μg/ml Peptide, recombinant protein Recombinant mouse CXCL1 R&D Systems Cat# 453- KC 10 ng/ml Peptide, recombinant protein Recombinant mouse IL- 1 beta R&D Systems Cat# 401ML- 005 40 ng/ml Peptide, recombinant protein RGD peptide Tocris Cat# 3498 10 mg/kg Peptide, recombinant protein CD31 agonist (P8RI) PMID:29957231 N/A 2.5 mg/kg Peptide, recombinant protein N- Formyl- Met- Leu- Phe Tocris Cat# 1921 1 μM Chemical compound, drug Viobility 405/520 fixable dye Miltenyi Biotec Cat# 130- 109- 816 1:100 Chemical compound, drug Alexa Fluor 647 Phalloidin Thermo Fisher Scientific Cat# A22287, RRID:AB_2620155 150 nM Chemical compound, drug DAPI Thermo Fisher Scientific Cat# D1306; RRID:AB_2629482 5 μM Chemical compound, drug Percoll Sigma Cat#P1644 Commercial assay or kit ProLong Gold Antifade Mountant Invitrogen Cat# P36930 Commercial assay or kit CellTrace Violet Cell Proliferation Kit Thermo Fisher Scientific Cat# C34571 Commercial assay or kit CellTrace CFSE Cell Proliferation Kit Thermo Fisher Scientific Cat# C34554 Commercial assay or kit EasySep Mouse Neutrophil Enrichment Kit Stem Cell Technologies Cat# 19762 Commercial assay or kit Mouse CXCL1/KC DuoSet ELISA R&D Systems Cat# DY453- 05 Commercial assay or kit Bio- Plex Pro Magnetic COOH Beads 26 Bio- Rad Cat# MC10026- 01 Commercial assay or kit Bio- Plex Amine Coupling Kit Bio- Rad Cat# 171406001 Commercial assay or kit Pierce BCA Protein Assay Kit Thermo Scientific Cat# 23225 Commercial assay or kit 10% Mini- PROTEAN TGX Precast Protein Gels, 10- well, 50 μl Bio- Rad Cat# 4561034 Commercial assay or kit Trans- Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Packs Bio- Rad Cat# 1704158 Commercial assay or kit Clarity Western ECL substrate kit Bio- Rad Cat# 1705060S Strain, strain background (Mus musculus) C57BL/6, C57BL/6 Charles River Internal colony Strain, strain background (M. musculus) Pecam1-/-, C57BL/6 PMID:10072554 Internal colony Strain, strain background (M. musculus) Lyz2- eGFP, C57BL/6 PMID:10887140 Internal colony Strain, strain background (M. musculus) Pecam1ITIM-/-, C57BL/6 Generated for this work Internal colony Continued Continued on next page Andreata, Clément et al. eLife 2023;12:e84752.

    Software:

    Article Title: Concurrence of FGFR1 mutations modulates oncogenesis in glioneuronal tumors.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK293 T-REx Flp-In (Human) Thermo Fisher #R78007 HOG (Human) Dr. Lopez Guerrero Lab MO3.13 (Human) Dr. Querol Gutierrez Lab Recombinant DNA pDONR-223 vector Addgene pcDNA5-FRT backbone vector Flippase-expressing plasmid (pOG44) PX458 vector Addgene #48138 Reagent/resource Reference or source Identifier or catalog number Antibodies Anti-FLAG Merck #F3165 Anti-FGFR1 Abcam #ab76464 Anti-phospho-FGFR1 Tyr653/ 654 Cell Signaling Technology #52928 Anti-PLCγ1 Cell Signaling Technology #5690 Anti-ERK1/2 Cell Signaling Technology #9102S Anti-phospho-ERK1/2 (Thr202/ Tyr204) Cell Signaling Technology #9101S Anti-phospho-PLCγ1 Tyr783 Cell Signaling Technology #2821 Anti-tubulin Merck #F6199 Streptavidin-HRP Thermofisher #21126 Anti-rabbit-HRP Merck #A0545 Anti-mouse-HRP Invitrogen #32430 Anti-mouse Alexa Fluor 488 Invitrogen #A21202 Anti-rabbit Alexa Fluor 555 Invitrogen #A21430 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S2 qPCR primers This study Appendix Table S5 gRNA sequences This study Appendix Table S3 Alt-RTM HDR Donor Oligos This study Appendix Table S4 Chemicals, enzymes and other reagents Doxycycline Merck #D9891 FGF2 RD Systems #233-FB Cycloheximide Merck C7698 Bafilomycin A1 Merck #B1793 Hygromycin B Merck #10843555001 Blasticidin Merck #SBR00022 Tetracycline Merck #T7660 Biotin Merck #B4501 Protein G Mag Sepharose beads Cytiva #28944008 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Prolong Gold Antifade Reagent with DAPI Invitrogen #P36935 Matrigel® Corning #354234 T7 endonuclease I New England Biolabs #M0302S AZD4547 (Fexagratinib) Astra Zeneca 1035270-39-3 Software R software v4.3.3 https://www.rproject.org/ ImageJ software https://imagej.net /ij/ SAINTexpress v3.6.1 Teo et al, 2014 AlphaFold 3 DeepMind STAR v2.7.0a limma package version 1.8.2 Bioconductor Other TransBlot Turbo RTA Transfer Kit Bio-RAD #1704158 Clarity Western ECL Substrate Kit Bio-RAD #1705060 BCA Protein Assay Kit ThermoScientific #23227 RNeasy Kit Qiagen #74104 SuperScript Reverse Transcriptase II Kit Invitrogen #18064014 PowerUp SYBR Green Master Mix Applied Biosystems #A25776 RNA 6000 Nano Assay Agilent #50671511 TruSeq® Stranded mRNA LT Sample Prep Kit Illumina #20020594 Review of public tumor data Public tumor data was interrogated using the GENIE/cBioportal database (de Bruijn et al, 2023) (https://genie.cbioportal.org/), selecting the GENIE Cohort v16.1 (last accessed in October 2024) and query for FGFR1 gene. ..

    Western Blot:

    Article Title: Concurrence of FGFR1 mutations modulates oncogenesis in glioneuronal tumors.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK293 T-REx Flp-In (Human) Thermo Fisher #R78007 HOG (Human) Dr. Lopez Guerrero Lab MO3.13 (Human) Dr. Querol Gutierrez Lab Recombinant DNA pDONR-223 vector Addgene pcDNA5-FRT backbone vector Flippase-expressing plasmid (pOG44) PX458 vector Addgene #48138 Reagent/resource Reference or source Identifier or catalog number Antibodies Anti-FLAG Merck #F3165 Anti-FGFR1 Abcam #ab76464 Anti-phospho-FGFR1 Tyr653/ 654 Cell Signaling Technology #52928 Anti-PLCγ1 Cell Signaling Technology #5690 Anti-ERK1/2 Cell Signaling Technology #9102S Anti-phospho-ERK1/2 (Thr202/ Tyr204) Cell Signaling Technology #9101S Anti-phospho-PLCγ1 Tyr783 Cell Signaling Technology #2821 Anti-tubulin Merck #F6199 Streptavidin-HRP Thermofisher #21126 Anti-rabbit-HRP Merck #A0545 Anti-mouse-HRP Invitrogen #32430 Anti-mouse Alexa Fluor 488 Invitrogen #A21202 Anti-rabbit Alexa Fluor 555 Invitrogen #A21430 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S2 qPCR primers This study Appendix Table S5 gRNA sequences This study Appendix Table S3 Alt-RTM HDR Donor Oligos This study Appendix Table S4 Chemicals, enzymes and other reagents Doxycycline Merck #D9891 FGF2 RD Systems #233-FB Cycloheximide Merck C7698 Bafilomycin A1 Merck #B1793 Hygromycin B Merck #10843555001 Blasticidin Merck #SBR00022 Tetracycline Merck #T7660 Biotin Merck #B4501 Protein G Mag Sepharose beads Cytiva #28944008 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Prolong Gold Antifade Reagent with DAPI Invitrogen #P36935 Matrigel® Corning #354234 T7 endonuclease I New England Biolabs #M0302S AZD4547 (Fexagratinib) Astra Zeneca 1035270-39-3 Software R software v4.3.3 https://www.rproject.org/ ImageJ software https://imagej.net /ij/ SAINTexpress v3.6.1 Teo et al, 2014 AlphaFold 3 DeepMind STAR v2.7.0a limma package version 1.8.2 Bioconductor Other TransBlot Turbo RTA Transfer Kit Bio-RAD #1704158 Clarity Western ECL Substrate Kit Bio-RAD #1705060 BCA Protein Assay Kit ThermoScientific #23227 RNeasy Kit Qiagen #74104 SuperScript Reverse Transcriptase II Kit Invitrogen #18064014 PowerUp SYBR Green Master Mix Applied Biosystems #A25776 RNA 6000 Nano Assay Agilent #50671511 TruSeq® Stranded mRNA LT Sample Prep Kit Illumina #20020594 Review of public tumor data Public tumor data was interrogated using the GENIE/cBioportal database (de Bruijn et al, 2023) (https://genie.cbioportal.org/), selecting the GENIE Cohort v16.1 (last accessed in October 2024) and query for FGFR1 gene. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Concurrence of FGFR1 mutations modulates oncogenesis in glioneuronal tumors.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK293 T-REx Flp-In (Human) Thermo Fisher #R78007 HOG (Human) Dr. Lopez Guerrero Lab MO3.13 (Human) Dr. Querol Gutierrez Lab Recombinant DNA pDONR-223 vector Addgene pcDNA5-FRT backbone vector Flippase-expressing plasmid (pOG44) PX458 vector Addgene #48138 Reagent/resource Reference or source Identifier or catalog number Antibodies Anti-FLAG Merck #F3165 Anti-FGFR1 Abcam #ab76464 Anti-phospho-FGFR1 Tyr653/ 654 Cell Signaling Technology #52928 Anti-PLCγ1 Cell Signaling Technology #5690 Anti-ERK1/2 Cell Signaling Technology #9102S Anti-phospho-ERK1/2 (Thr202/ Tyr204) Cell Signaling Technology #9101S Anti-phospho-PLCγ1 Tyr783 Cell Signaling Technology #2821 Anti-tubulin Merck #F6199 Streptavidin-HRP Thermofisher #21126 Anti-rabbit-HRP Merck #A0545 Anti-mouse-HRP Invitrogen #32430 Anti-mouse Alexa Fluor 488 Invitrogen #A21202 Anti-rabbit Alexa Fluor 555 Invitrogen #A21430 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S2 qPCR primers This study Appendix Table S5 gRNA sequences This study Appendix Table S3 Alt-RTM HDR Donor Oligos This study Appendix Table S4 Chemicals, enzymes and other reagents Doxycycline Merck #D9891 FGF2 RD Systems #233-FB Cycloheximide Merck C7698 Bafilomycin A1 Merck #B1793 Hygromycin B Merck #10843555001 Blasticidin Merck #SBR00022 Tetracycline Merck #T7660 Biotin Merck #B4501 Protein G Mag Sepharose beads Cytiva #28944008 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Prolong Gold Antifade Reagent with DAPI Invitrogen #P36935 Matrigel® Corning #354234 T7 endonuclease I New England Biolabs #M0302S AZD4547 (Fexagratinib) Astra Zeneca 1035270-39-3 Software R software v4.3.3 https://www.rproject.org/ ImageJ software https://imagej.net /ij/ SAINTexpress v3.6.1 Teo et al, 2014 AlphaFold 3 DeepMind STAR v2.7.0a limma package version 1.8.2 Bioconductor Other TransBlot Turbo RTA Transfer Kit Bio-RAD #1704158 Clarity Western ECL Substrate Kit Bio-RAD #1705060 BCA Protein Assay Kit ThermoScientific #23227 RNeasy Kit Qiagen #74104 SuperScript Reverse Transcriptase II Kit Invitrogen #18064014 PowerUp SYBR Green Master Mix Applied Biosystems #A25776 RNA 6000 Nano Assay Agilent #50671511 TruSeq® Stranded mRNA LT Sample Prep Kit Illumina #20020594 Review of public tumor data Public tumor data was interrogated using the GENIE/cBioportal database (de Bruijn et al, 2023) (https://genie.cbioportal.org/), selecting the GENIE Cohort v16.1 (last accessed in October 2024) and query for FGFR1 gene. ..

    Reverse Transcription:

    Article Title: Concurrence of FGFR1 mutations modulates oncogenesis in glioneuronal tumors.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK293 T-REx Flp-In (Human) Thermo Fisher #R78007 HOG (Human) Dr. Lopez Guerrero Lab MO3.13 (Human) Dr. Querol Gutierrez Lab Recombinant DNA pDONR-223 vector Addgene pcDNA5-FRT backbone vector Flippase-expressing plasmid (pOG44) PX458 vector Addgene #48138 Reagent/resource Reference or source Identifier or catalog number Antibodies Anti-FLAG Merck #F3165 Anti-FGFR1 Abcam #ab76464 Anti-phospho-FGFR1 Tyr653/ 654 Cell Signaling Technology #52928 Anti-PLCγ1 Cell Signaling Technology #5690 Anti-ERK1/2 Cell Signaling Technology #9102S Anti-phospho-ERK1/2 (Thr202/ Tyr204) Cell Signaling Technology #9101S Anti-phospho-PLCγ1 Tyr783 Cell Signaling Technology #2821 Anti-tubulin Merck #F6199 Streptavidin-HRP Thermofisher #21126 Anti-rabbit-HRP Merck #A0545 Anti-mouse-HRP Invitrogen #32430 Anti-mouse Alexa Fluor 488 Invitrogen #A21202 Anti-rabbit Alexa Fluor 555 Invitrogen #A21430 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S2 qPCR primers This study Appendix Table S5 gRNA sequences This study Appendix Table S3 Alt-RTM HDR Donor Oligos This study Appendix Table S4 Chemicals, enzymes and other reagents Doxycycline Merck #D9891 FGF2 RD Systems #233-FB Cycloheximide Merck C7698 Bafilomycin A1 Merck #B1793 Hygromycin B Merck #10843555001 Blasticidin Merck #SBR00022 Tetracycline Merck #T7660 Biotin Merck #B4501 Protein G Mag Sepharose beads Cytiva #28944008 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Prolong Gold Antifade Reagent with DAPI Invitrogen #P36935 Matrigel® Corning #354234 T7 endonuclease I New England Biolabs #M0302S AZD4547 (Fexagratinib) Astra Zeneca 1035270-39-3 Software R software v4.3.3 https://www.rproject.org/ ImageJ software https://imagej.net /ij/ SAINTexpress v3.6.1 Teo et al, 2014 AlphaFold 3 DeepMind STAR v2.7.0a limma package version 1.8.2 Bioconductor Other TransBlot Turbo RTA Transfer Kit Bio-RAD #1704158 Clarity Western ECL Substrate Kit Bio-RAD #1705060 BCA Protein Assay Kit ThermoScientific #23227 RNeasy Kit Qiagen #74104 SuperScript Reverse Transcriptase II Kit Invitrogen #18064014 PowerUp SYBR Green Master Mix Applied Biosystems #A25776 RNA 6000 Nano Assay Agilent #50671511 TruSeq® Stranded mRNA LT Sample Prep Kit Illumina #20020594 Review of public tumor data Public tumor data was interrogated using the GENIE/cBioportal database (de Bruijn et al, 2023) (https://genie.cbioportal.org/), selecting the GENIE Cohort v16.1 (last accessed in October 2024) and query for FGFR1 gene. ..

    SYBR Green Assay:

    Article Title: Concurrence of FGFR1 mutations modulates oncogenesis in glioneuronal tumors.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK293 T-REx Flp-In (Human) Thermo Fisher #R78007 HOG (Human) Dr. Lopez Guerrero Lab MO3.13 (Human) Dr. Querol Gutierrez Lab Recombinant DNA pDONR-223 vector Addgene pcDNA5-FRT backbone vector Flippase-expressing plasmid (pOG44) PX458 vector Addgene #48138 Reagent/resource Reference or source Identifier or catalog number Antibodies Anti-FLAG Merck #F3165 Anti-FGFR1 Abcam #ab76464 Anti-phospho-FGFR1 Tyr653/ 654 Cell Signaling Technology #52928 Anti-PLCγ1 Cell Signaling Technology #5690 Anti-ERK1/2 Cell Signaling Technology #9102S Anti-phospho-ERK1/2 (Thr202/ Tyr204) Cell Signaling Technology #9101S Anti-phospho-PLCγ1 Tyr783 Cell Signaling Technology #2821 Anti-tubulin Merck #F6199 Streptavidin-HRP Thermofisher #21126 Anti-rabbit-HRP Merck #A0545 Anti-mouse-HRP Invitrogen #32430 Anti-mouse Alexa Fluor 488 Invitrogen #A21202 Anti-rabbit Alexa Fluor 555 Invitrogen #A21430 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S2 qPCR primers This study Appendix Table S5 gRNA sequences This study Appendix Table S3 Alt-RTM HDR Donor Oligos This study Appendix Table S4 Chemicals, enzymes and other reagents Doxycycline Merck #D9891 FGF2 RD Systems #233-FB Cycloheximide Merck C7698 Bafilomycin A1 Merck #B1793 Hygromycin B Merck #10843555001 Blasticidin Merck #SBR00022 Tetracycline Merck #T7660 Biotin Merck #B4501 Protein G Mag Sepharose beads Cytiva #28944008 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Prolong Gold Antifade Reagent with DAPI Invitrogen #P36935 Matrigel® Corning #354234 T7 endonuclease I New England Biolabs #M0302S AZD4547 (Fexagratinib) Astra Zeneca 1035270-39-3 Software R software v4.3.3 https://www.rproject.org/ ImageJ software https://imagej.net /ij/ SAINTexpress v3.6.1 Teo et al, 2014 AlphaFold 3 DeepMind STAR v2.7.0a limma package version 1.8.2 Bioconductor Other TransBlot Turbo RTA Transfer Kit Bio-RAD #1704158 Clarity Western ECL Substrate Kit Bio-RAD #1705060 BCA Protein Assay Kit ThermoScientific #23227 RNeasy Kit Qiagen #74104 SuperScript Reverse Transcriptase II Kit Invitrogen #18064014 PowerUp SYBR Green Master Mix Applied Biosystems #A25776 RNA 6000 Nano Assay Agilent #50671511 TruSeq® Stranded mRNA LT Sample Prep Kit Illumina #20020594 Review of public tumor data Public tumor data was interrogated using the GENIE/cBioportal database (de Bruijn et al, 2023) (https://genie.cbioportal.org/), selecting the GENIE Cohort v16.1 (last accessed in October 2024) and query for FGFR1 gene. ..

    Sample Prep:

    Article Title: Concurrence of FGFR1 mutations modulates oncogenesis in glioneuronal tumors.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HEK293 T-REx Flp-In (Human) Thermo Fisher #R78007 HOG (Human) Dr. Lopez Guerrero Lab MO3.13 (Human) Dr. Querol Gutierrez Lab Recombinant DNA pDONR-223 vector Addgene pcDNA5-FRT backbone vector Flippase-expressing plasmid (pOG44) PX458 vector Addgene #48138 Reagent/resource Reference or source Identifier or catalog number Antibodies Anti-FLAG Merck #F3165 Anti-FGFR1 Abcam #ab76464 Anti-phospho-FGFR1 Tyr653/ 654 Cell Signaling Technology #52928 Anti-PLCγ1 Cell Signaling Technology #5690 Anti-ERK1/2 Cell Signaling Technology #9102S Anti-phospho-ERK1/2 (Thr202/ Tyr204) Cell Signaling Technology #9101S Anti-phospho-PLCγ1 Tyr783 Cell Signaling Technology #2821 Anti-tubulin Merck #F6199 Streptavidin-HRP Thermofisher #21126 Anti-rabbit-HRP Merck #A0545 Anti-mouse-HRP Invitrogen #32430 Anti-mouse Alexa Fluor 488 Invitrogen #A21202 Anti-rabbit Alexa Fluor 555 Invitrogen #A21430 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S2 qPCR primers This study Appendix Table S5 gRNA sequences This study Appendix Table S3 Alt-RTM HDR Donor Oligos This study Appendix Table S4 Chemicals, enzymes and other reagents Doxycycline Merck #D9891 FGF2 RD Systems #233-FB Cycloheximide Merck C7698 Bafilomycin A1 Merck #B1793 Hygromycin B Merck #10843555001 Blasticidin Merck #SBR00022 Tetracycline Merck #T7660 Biotin Merck #B4501 Protein G Mag Sepharose beads Cytiva #28944008 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalog number Prolong Gold Antifade Reagent with DAPI Invitrogen #P36935 Matrigel® Corning #354234 T7 endonuclease I New England Biolabs #M0302S AZD4547 (Fexagratinib) Astra Zeneca 1035270-39-3 Software R software v4.3.3 https://www.rproject.org/ ImageJ software https://imagej.net /ij/ SAINTexpress v3.6.1 Teo et al, 2014 AlphaFold 3 DeepMind STAR v2.7.0a limma package version 1.8.2 Bioconductor Other TransBlot Turbo RTA Transfer Kit Bio-RAD #1704158 Clarity Western ECL Substrate Kit Bio-RAD #1705060 BCA Protein Assay Kit ThermoScientific #23227 RNeasy Kit Qiagen #74104 SuperScript Reverse Transcriptase II Kit Invitrogen #18064014 PowerUp SYBR Green Master Mix Applied Biosystems #A25776 RNA 6000 Nano Assay Agilent #50671511 TruSeq® Stranded mRNA LT Sample Prep Kit Illumina #20020594 Review of public tumor data Public tumor data was interrogated using the GENIE/cBioportal database (de Bruijn et al, 2023) (https://genie.cbioportal.org/), selecting the GENIE Cohort v16.1 (last accessed in October 2024) and query for FGFR1 gene. ..



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